《植物生理学报》 2010, 46(5): 423-426

硝普钠、植酸和水杨酸对悬浮培养的霍山石斛原球茎生长和生理活性的

黄蓓, 洪萨丽, 金青, 张士鸿, 蔡永萍*, 林毅

安徽农业大学生命科学学院, 合肥230036

通信作者：蔡永萍；E-mail: swkx12@ahau.edu.cn；Tel: 0551-5786232

摘　要：

在建立霍山石斛的液体悬浮培养技术的基础上, 添加诱导子硝普钠(SNP)、植酸(PA)和水杨酸(SA)。3 种诱导子均可 促进霍山石斛原球茎中生物碱的积累, 0.1 mmol•L-1 SNP、7.5 g•L-1 PA 和 100 μmol•L-1 SA 处理的原球茎, 其生物碱含量分别 为不作处理的 2.02 倍、1.84 倍和 1.62 倍。促进霍山石斛生物碱积累的适宜诱导子为 SNP, 其适宜浓度为 0.1 mmol•L-1。高 浓度的3种诱导子均导致培养液酸化, 原球茎的相对电导率上升, 其生物量和生物碱含量明显下降。

关键词：霍山石斛; 原球茎; 悬浮培养; 生物碱; 诱导子

收稿：2009-11-30   修定：2010-03-05

资助：安徽省教育厅重点项目(2006KJ054A)和安徽省教育厅十五人才基金。

Effects of SNP, PA and SA on Cell Growth and Physiological Activities of Suspension-Cultured Protocorn-Like Bodies of Dendrobium huoshanense C. Z.Tang et S. J. Cheng

HUANG Bei, HONG Sa-Li, JIN Qing, ZHANG Shi-Hong, CAI Yong-Ping*, LIN Yi

School of Life Sciences, Anhui Agricultural University, Hefei 230036, China

Corresponding author: CAI Yong-Ping; E-mail: swkx12@ahau.edu.cn; Tel: 0551-5786232

Abstract:

It was studied the influence of sodium nitroprusside (SNP), phytic acid (PA) and salicylic acid (SA) on cell growth and the alkaloid accumulation of suspension-cultured protocorn-like bodies (PLBs) of Dendrobium huoshanense. The results showed that all the three elicitors could enhance the accumulation of alkaloid. When treated with 0.1 mmol·L^-1 SNP, 7.5 g·L^-1 PA, and 100 μmol·L^-1 SA, respectively, the alkaloids accumulation elevated to 2.02-, 1.84-, and 1.62-fold compared with control. The appropriate elicitor was SNP, whose optimum concentration was 0.1 mmol·L^-1. High concentration of three elicitors could cause the medium acidification and increase the relative conductivity of PLBs, resulted in reduced biomass and alkaloids quantity.

Key words: Dendrobium huoshanense; protocorm-like bodies; suspension culture; alkaloids; elicitor